Spider-Man Vol 1 #45

 Pursuit Part 1 of 4.

"The Dream Before"

Following the tragic events of Amazing Spider-Man 388, the web-slinger vows revenge on the Chameleon! But before he can go after the faceless villain full bore, he must tell Aunt May -- that Mary and Richard Parker are gone.

Surprisingly, May Parker takes the news well, because she's had her suspicions about them all along. So with the family business complete, it's time for Spider-Man to crush the Chameleon!

Story continues in Spectacular Spider-Man 211.

Cameo appearance by Mary Jane Watson.